General Cell Based Assays
Eurofins Discoverx PathHunter eXpress IL12RB1/IL12RB2 Dimerization Assay, 2pl Kit. (93-1041E3CP0M)
The PathHunter IL12RB1/IL12RB2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEDNRA CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0685E2MCP0L)
The PathHunter mEDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of mEDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRA and EA-tagged β-Arrestin-2. Activation of mEDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PRLHR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0302E2CP2M)
The PathHunter PRLHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PrRP) based activation of PRLHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PRLHR and EA-tagged β-Arrestin-2. Activation of PRLHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Estradiol 3-sulfate 17β-Glucuronide (potassium salt), Purity: ≥95%, CAS: 10392-35-5, 5 mg
A metabolite of estradiol 3-sulfate that has been modified by a UDP-glucuronosyltransferase; enhances utilization by anion transporters, most commonly leading to excretion in urine and bile
Eurofins Discoverx PathHunter Mouse P2RY6 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Mouse P2RY6 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure mP2RY6 (GPCR) activity via recruitment of β-Arrestin2. Mouse protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical GGT Substrate Diluent, 3.5 mL
GGT substrate diluent has been tested and formulated to work exclusively with Cayman’s γ-Glutamyl Transferase Activity Assay Kit (Item No. 702210).
Eurofins Discoverx cAMP Hunter eXpress ADRB2 (B2AR) CHO-K1 GPCR Assay, 10-Plate Kit. (95-0121E2CP2L)
The cAMP Hunter ADRB2 (B2AR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB2, a naturally Gs coupled GPCR. Upon activation of ADRB2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences CV-Cathepsin K detection kit (1 Kit)
Elevated cathepsin enzyme activity often indicates the presence of a pathological condition including cancer, Alzheimer's disease, autoimmune disease, and osteoporosis. Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy. Once cells are grown, staining is completed in just a few minutes. Hoechst 33342 stain and Acridine Orange are also included to allow visualization of nuclei and lysosomes. Applications: Fluorescence microscopy, Fluorescent detection .
Eurofins Discoverx PathHunter eXpress GPR141 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0385E2ACP1L)
The PathHunter GPR141 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mUTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0525E2MCP0M)
The PathHunter mUTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. urotensin II) based activation of mUTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mUTR2 and EA-tagged β-Arrestin-2. Activation of mUTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Human Adenosine A1 Receptor (AA1R, ADORA1) Big Sky BacMam, 15mL
Human A1 Adenosine receptor packaged in Montana Molecular's Big Sky BacMam vector. Express the adenosine receptor in your cells of interest. Big Sky BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Eurofins Discoverx PathHunter eXpress AVPR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0588E2CP0L)
The PathHunter AVPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR2 and EA-tagged β-Arrestin-2. Activation of AVPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mVIPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0619E2MCP2M)
The PathHunter mVIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of mVIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mVIPR1 and EA-tagged β-Arrestin-2. Activation of mVIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRB1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0488E2CP0L)
The PathHunter ADRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB1 and EA-tagged β-Arrestin-2. Activation of ADRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.